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Automated transient protein expression—
Expi293™ system on the ambr™ system

3,324 views · Published 18 April 2013 · 3:41 · Indexed 27 October 2026

Channel: Thermo Fisher Scientific · 2013 · Science & Technology

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Learn how the breakthrough Expi293 Expression System can be combined with the TAP Biosystems ambr™ Advanced Micro Bioreactor System to provide major advantages in transient protein expression. The increases in consistency and productivity inherent in both systems multiply to deliver a highly efficient automated cell culture platform for small-scale transient protein expression.

When we first started developing the Expi293 we talked to a lot of customers, mainly in the industrial arena. And a lot of them were looking for a good solution for taking the mammalian proteins and expressing them at smaller scales in a high throughput form.

Our goals in establishing Expi on the ambr platform were really to ensure from an R&D standpoint that our product, the EXpi293, maintained its robustness, its high level of protein expression and the quality of protein in the process of automation.

Well I'm very excited personally about the combination of the ambr and the Expi system, really the enhancements in protein productivity and the consistency and quality that are delivered by the Expi system rally have a great synergy with the automation oand the stability of control on the platform on the ambr to reduce the amount of scientist time required to support a wide range of screening experiments.

The advantages of Expi293 are that the system was designed to give customers really robust protein expression -- far more protein than they are able to get with some of the systems that they are currently using. But also the system is incredibly reproducible both run to run and experiment to experiment.

Ambr is an automated micro-bioreactor system including a micro-bioreactor vessel and automated workstation for the maintenance of up to 24 or 48 animal cell cultures in parallel. This really enables scientists to carry out a range of screening experiments perhaps looking at different cell lines, different process conditions but also different proteins or DNA constructs as well. The synergy of the ambr and the Expi293 is really based around supporting the process control conditions that the ambr can provide. It gives a really solid basis for the comparison of performance in a range of these different parallel experiments.

What I like about the ambr system is really that for one thing is a small platform, its easy to fit in everybody's tissue culture hood so that makes it easy to use. But really it's the only solution out there that does act as a complete cell culture system, so its unique for our customers. Although Expi is already a very easy to use system, by automating it you can eliminate that extra bit of user variability and error. I think these two things -- by being able to do more experiments and eliminate error will really resonate with our customers who are interested in high throughput applications.

The use of the ambr with Expi293 is likely to be of great interest to scientists who are carrying out smalle scale and medium scale parallel experiments, screening experiments around about the 30ml scale of culture. Anyone who is looking at a range of different process conditions, different protein expression, different DNA constructs -- the ambr will e very useful to them because it can automate the set up and maintenance of these cultures, reducing the amount of time.

So, by combining Expi and ambr I think this is going to be a great solution for them

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