Aneously firing neurons observed using FluoVolt™ Membrane Potential Dye
2,616 views · Published 22 August 2014 · 0:05 · Indexed 30 October 2026
Channel: Thermo Fisher Scientific · 2014 · Science & Technology
http://www.lifetechnologies.com/us/en/home/life-science/cell-analysis/cell-viability-and-regulation/ion-indicators/membrane-potential-indicators.html#1 Cholinergic NG-108 neuronal cells will fire spontaneously in culture when differentiated with dibutyryl cyclic AMP. Here, their asynchronous discharges are observed as fluorescent signals in real time using FluoVolt™ Membrane Potential Dye to monitor excitability. NG-108 cells (from ATCC) were cultured and differentiated according to published protocols, then loaded with FluoVolt™ Membrane Potential Dye for 15 minutes at room temperature in Live Cell Imaging Solution (LCIS) with 10 mM glucose. After loading, the dye was removed and replaced with LCIS with 10 mM glucose. Cultures were imaged with 5 ms illumination pulses at 10 ms intervals using a Till Photonics (FEI Life Sciences) Polychrome V monochromator and images acquired with 2x binning. Asynchronous firing between the cells can be clearly seen in the time course of this measurement.
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