Time course of cell death visualized using ReadyProbes™ reagents.
1,431 views · Published 5 August 2014 · 0:16 · Indexed 6 November 2026
Channel: Thermo Fisher Scientific · 2014 · Science & Technology
Time course of cell death visualized using ReadyProbes™ reagents. A549 cells were pre-treated with NucGreen™ Dead 488 (R37109) and NucRed™ Live 647 (R37106) ReadyProbes™ reagents to stain living cells red and dead cells green. A Mat-Tek dish of cells was preloaded with 2 drops per mL of ReadyProbe™ reagents in complete medium reagents and placed on a Deltavision™ Core microscope. Cell death was achieved by the addition of Triton® X-100 to one side of the dish for a final concentration of 0.2%. DIC, green, red channel were collected over a 15 minute period using a 40X objective.
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