Viability determination of HeLa cells using ReadyProbes® Cell Viability Imaging Kit (Blue/Red)
1,796 views · Published 11 March 2014 · 0:10 · Indexed 10 November 2026
Channel: Thermo Fisher Scientific · 2014 · Science & Technology
Learn more at http://www.lifetechnologies.com/readyprobes HeLa cells were loaded with NucBlue® Live and PI (using 2 drops per ml) in complete media for 15 minutes at 37oC. Cells were treated with DMSO vehicle control for staurosporine and images were acquired every 30 min. for 18 hours using EVOS® Auto Imaging System. All cells are stained with NucBlue® Live, shown with blue nuclei. Over time an increase in the number of dead cells is observed as indicated by green nuclei (PI).
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