1.5 Autofluorescence–Fixed cell imaging: 5 steps for publication-quality images
4,168 views · Published 5 February 2018 · 4:33 · Indexed 19 October 2026
Channel: Thermo Fisher Scientific · 2018 · Science & Technology
https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/fixed-cell-imaging-reagents.html?cid=BID_R01_PJT3278_BID88888_VI_YUT_OD_KT_065 The last step in cell preparation is autofluorescence. Cells and tissue can have a certain degree of autofluorescence that can confuse the specific signal, and lower the signal-to-background. Overcoming autofluorescence means greater sensitivity.
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