Z Stack image of HeLa cells labeled with CellLightsTM Reagents
2,139 views · Published 5 August 2014 · 0:06 · Indexed 19 October 2026
Channel: Thermo Fisher Scientific · 2014 · Science & Technology
A series of images were captured on the EVOS FL Auto Cell Imaging System. Creating a Z-stack from these images allowed the observation of cellular cytoskeletal changes, which can be indicative of the loss of cell health. Methods HeLa cells grown in MatTek 6-well glass bottom culture plates were transduced with CellLights™ Tubulin-GFP and CellLight™ Mitochondria-RFP overnight at 37oC. The following day, NucBlue Live reagent (2 drops/mL) was added to the cultures. Cells were then imaged on an EVOS FL Auto Cell Imaging System with 100x oil immersion objective using the Z-stack function. The step size was set using the Nyquist formula and performed at 0.366μm.
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