2.2 Primary antibody protocol optimization:Fixed cell imaging:5 steps for publication quality images
1,017 views · Published 22 March 2018 · 4:36 · Indexed 25 October 2026
Channel: Thermo Fisher Scientific · 2018 · Science & Technology
https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/fixed-cell-imaging-reagents.html?cid=BID_R01_PJT3278_BID88888_VI_YUT_OD_KT_047 Every primary antibody must be optimized separately. There are many protocols available, and it is important to understand a “one size fits all” approach gives inferior results, as every antibody is slightly different.
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