Detection of oxidative stress with CellROX Orange in U2 OS cells
3,853 views · Published 5 August 2014 · 0:07 · Indexed 14 November 2026
Channel: Thermo Fisher Scientific · 2014 · Science & Technology
U2-OS cells were plated at 200,000 cells per dish on a 35 mm glass bottom dish (MatTek) and cultured overnight. The cells were rinsed once and loaded with 5 µM CellROX® Orange Reagent (Catalog # C10443) in Live Cell Imaging Solution (LCIS, Cat #A14291DJ) for 15 minutes at 37 degrees C. After loading, cells were imaged live with no washing every 20 seconds for seventy minutes on the DeltaVision Core™ inverted microscope at 37 degrees C. TBHP was added to a final concentration of 200 uM after ten minutes of baseline acquisition. To learn more, please visit: http://products.invitrogen.com/ivgn/product/C10443
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