Look Who it Was Random video

Dr. Glen Reid loves Lipofectamine® RNAiMAX

2,295 views · Published 11 September 2013 · 2:29 · Indexed 10 November 2026

Channel: Thermo Fisher Scientific · 2013 · Science & Technology

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"Based on our experience using Lipofectamine® [reagents] over a number of years, we definitely [recommend] it to colleagues and people interested in transfection....Firstly, it is very easy to use [and] very scalable, and also it is extremely nontoxic...so for those two reasons alone Lipofectamine® RNAiMAX for siRNAs and microRNA mimics is a very useful and recommendable reagent for transfection." 

—Dr. Glen Reid, Sydney, Australia

(What is your area of research?)

My main area of research is the disease malignant mesothelioma. This is a disease of the lining of the lungs caused by asbestos exposure. We are looking at using an RNAi-based screening approach to identify new targets and we are also looking at micro RNA expression profiles to see whether we can identify micro RNAs that might be useful as therapeutic targets in the future.

(What kind of cells do you transfect?)

The cells we typically transfect here in the lab are those derived from tumors from mesothelioma patients. These tend to be those cells that grow in an inherent culture.

(Why do you choose Lipofectamine® reagents for your transfection experiments?)

For all experiments we've been doing currently we've been using Lipofectamine® RNA Max. The main reasons that we use this reagent are two-fold; one is that it is extremely easy to use and also scalable meaning that we can transfect equally well in a 96-well plate as well as say a 6-well or even a 325 flask. Also, its extremely non-toxic, we find that very helpful for ourselves we don't get any off target effects due to the toxicity of the Lipofectamine® reagent and also we find that oftentimes we don't have to change the transfection medium.


(What do you like best about them?)

So, in our experiments using Lipofectamine® RNAi Max to support our RNAi based screening we are very happy to be able to go through quite a number of cell lines, quite a number of gene targets in a relatively quick time and because Lipofectamine® RNA Max in our hands works really well in 96-well reverse transfection experiments we quickly came to a very good set of candidate genes for the investigation.


(Would you recommend Lipofectamine® to your colleagues?)

We definitely it to colleagues and people interested in transfection and again this is based on the reasons why we like it ourselves. First that it is very easy to use, very scalable and also that it is extremely non-toxic, at least in our hands.

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