Phase-Contrast Imaging of a Wound-Healing Experiment Using HBECs

829 views · Published 15 December 2015 · 0:10 · Indexed 8 November 2026

Channel: Cell Press · 2015 · Science & Technology

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The wound-healing response of human bronchial epithelial cells (HBECs) after a monolayer was scratched was imaged by phase-contrast microscopy (left) and computationally analyzed (stepwise protrusion from early steps [dark blue] to late steps [dark red], right). These data, along with other analyses of the wound-healing dynamics and traction stresses, allowed Zaritsky et al. to propose a mechanism by which velocity and force alignment coordinate the collective movement of the cell sheet. See the article by Zaritsky et al. in the December 15 issue of Biophysical Journal.

Read more at http://www.cell.com/biophysj/abstract/S0006-3495(15)01123-6

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