Self-Forming Retina
1,187 views · Published 7 December 2018 · 0:16 · Indexed 1 November 2026
Channel: Cell Press · 2018 · Science & Technology
For nearly 100 years, biologists have debated about what causes the deformation of the optic vesicle epithelium, with many speculating that forces and/or signals from surrounding tissues (e.g., the lens) drive optic vesicle invagination. This movie shows the optic cup spontaneously forming form embryonic stem cells in a 3D culture devoid of lens or surface ectoderm, demonstrating that a self-organizing, intrinsic mechanism drives optic vesicle invagination. The first part of the video shows bright field images (left) and optical section images taken with multi-photon microscopy (right; green, Rx::GFP for retinal marking) during culture days 7-9. The distal portion of the evaginated optic vesicle, which gives rise to neural retina, undergoes a dynamic invagination. The outer shell of the cup structure develops into retinal pigment epithelium while the inner shell gives rise to the retina. The second part of the movie shows a 3D reconstruction of optic cup's surface. Learn more in Eiraku and Sasai (2011) (http://www.nature.com/nature/journal/v472/n7341/full/nature09941.html). Video courtesy of Mototsugu Eiraku and Yoshiki Sasai, RIKEN. See more vision images in the Cell Picture Show: https://www.cell.com/pictureshow/vision
More from this channel
-
2:55
Chromatin shapes the mitotic spindle
-
5:14
The architecture of adaptation
-
4:14
Wnts come in small packages
-
6:09
Helicases Find Their Way
-
3:16
Fighting Mosquito-Borne Infections
-
4:29
Leaving Trash with Moms Keeps Daughters Young
-
4:07
Feedback Control of Vocal Learning
-
4:22
DISC1 knockdown leads to behavioral deficits