In Vivo SynapGCaMP6f Imaging Can Detect Spontaneous Quantal Events at Both Ib and Is NMJs

115 views · Published 5 October 2017 · 0:15 · Indexed 10 November 2026

Channel: Cell Press · 2017 · Science & Technology

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Example of the in vivo automatic detection of spontaneous quantal transmission. Analysis of spontaneous release was performed between motor neuron activity bouts. Raw data on the top was acquired with an imaging frequency of 14 Hz and movie playback is at 20 FPS. Scale bar is 10 μm. The locations of identified and validated spontaneous release events are indicated with a green circle that appears right before, during, and after the peak of the quantal response. The bottom movie is an overlay of the ΔF/F signal for the identified spontaneous quantal events on the basal SynapGCaMP6f fluorescence at each frame.

Read more from Newman et al. (2017) in Neuron: http://www.cell.com/neuron/fulltext/S0896-6273(17)30136-8

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